Determination of the selectivity of kinase inhibitors is crucial to avoid side-effects caused by off-target inhibition. Biochemical profiling using a large panel of kinase activity assays with a broad coverage of the human kinome is essential for the assessment of selectivity and potential side-effects.

ProQinase biochemical kinase testing services currently comprises a panel of  358  recombinant human protein kinases and 10 lipid kinases for broad selectivity profiling of your compounds. With our two standardized service packages Kinase WholePanelProfiler (358 protein kinases) and  Kinase WholePanelProfiler^Plus (358 protein kinases & 10 lipid kinases) you have the opportunity to test a single or more compounds against a group of kinases covering about 60% of the human kinome, as recently published in Nature Chemical Biology¹.

Important features of our service:

• Two data points per cpd: 1 conc. in duplicate or 2 conc. in singlicate
• Assay technologies: Radiometric assay for all protein kinases and ADP-Glo^TM  kinase assay (Promega) for all lipid kinases 
• Testing at app. ATP K_m: With a few exceptions each kinase will be tested at the respective assay specific ATP K_m
• Rapid turnover: 10 working days maximally are guaranteed  

A quote and detailed information about submission of test samples will be provided upon request. Please inquire!

1: Koeberle SC, Romir J, Fischer S, Koeberle A, Schattel V, Albrecht W, Grütter C, Werz O, Rauh D, Stehle & Laufer SA (2011): Skepinone-L is a selective p38 mitogen-activated protein kinase inhibitor. Nature Chemical Biology 761 advance online publication

 
Profile Examples - Vatalanib and Sunitinib
³³PanQinase Assay® selectivity profile of Vatalanib (on top) and Sunitinib (below) at 3 uM each

List of kinases used for the service packages Kinase WholePanelProfiler and Kinase WholePanelProfiler^Plus

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