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ProQinases products & services are designed to support drug discovery for targeting cancer with a focus on protein kinases. |
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| In Vivo Testing Services | | ProQinase has established a large panel of subcuteanous xenografts and orthotopic tumor models for in vivo
testing. All orthotopic tumor studies are performed with luciferase
transfected tumor cell lines allowing bioluminescene imaging. Moreover,
metastasis tumor models are available for in vivo testing of lead compounds. ProQinase's unique spheroid-based in vivo angiogenesis assay allows in vivo testing of the anti-angiogenic effect of compounds or antibodies against functional human blood vessels in mice. | | | | Cellular Assay Services | ProQinase offers several types of cell-based assays to characterize
compounds on the cellular level for targeting cancer. On the one hand,
we offer cellular phosphorylation assays
to assess the inhibitory effect of compounds on a specific kinase
(currently established for 22 different kinases). On the other hand, we
offer various sophisticated phenotypic cell-based assays, such as (i)
the spheroid-based angiogenesis assay to evaluate the angiogenic or anti-angiogenic potential of compounds, (ii) the cell migration assay which allows to identify the impact of compounds on the motility of various cell lines, (iii) the cell proliferation assay to investigate the effect on tumor cell growth and (iv) the soft agar assay (or clonogenic assay) which characterizes compounds for their ability to inhibit cell transformation. | | | | Biochemical Kinase Assay Services | ProQinase offers different types of compound testing in biochemical
kinase tests with protein kinases and lipid kinases. Our highly flexible
free choice kinase assay projects
include high throughput screening (HTS) of large compound libraries
against one target, and selectivity profiling against several or all
kinases which are available at ProQinase (371 protein kinases and 13
lipid kinases). Kinase tests can be performed either as single
concentration measurements or as IC50 determinations. Moreover, ProQinase offers to test your compound(s) in predefined Kinase MutantProfiler
comprising different mutants and the wildtyp form of a given kinase
and including a side-by-side comparison with reference inhibitors.
The Kinase SubstrateFinder
is another standardized service package that allows identification of
protein kinase substrates for biochemical protein kinase activity
assays. The HitDetector is a service package which comprises biochemical testing of a compound library against a selected protein kinase. | | |
| Recombinant Proteins |
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ProQinase sells in-house produced recombinant kinases for enzymatic kinase tests. Currently 255 active protein kinases (including 69 mutant kinases and 11 active lipid kinases are available as off-the-shelf products. In addition, additional protein kinase variants (inactive or non-activated) are available from ProQinase. Kinases can also be produced as custom-tailored proteins according to your requirements. Moreover, ProQinase offers recombinant substrates for biochemical kinase activity assays. |
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Individual service packages fitting your individual needs are available on request. |
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